Catalog Number | LT8918 |
Product Name | Tyrosine Kinase Peptide 1, 5-TMR Labeled |
Product Quantity | 1 mg |
Sequence | 5-TMR-KVEKIGEGTYGVVYK |
Molecular Weight | 2082.5 |
Formula | C106H148ClN21O29 |
Purity | ≥95% |
Scientific Background | 5-TMR-KVEKIGEGTYGVVYK is a tetramethylrhodamine-labeled derivative of Tyrosine Kinase Peptide 1. The KVEKIGEGTYGVVYK sequence is derived from a CDC2-related region and has been used as a generic substrate for multiple protein tyrosine kinases. The N-terminal 5-TMR/TAMRA fluorophore enables direct fluorescence detection while retaining the peptide's tyrosine phosphorylation sites. |
Research Applications | - fluorescent protein tyrosine kinase assays
- kinase substrate profiling
- phosphorylation method development
- fluorescence-based inhibitor screening
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Experimental Notes | The 5-TMR label changes charge, hydrophobicity and chromatographic behavior compared with the unlabeled peptide. Protect from light and use assay-specific kinetic controls. |
Selected References | - Identification and characterization of a novel synthetic peptide substrate specific for Src-family protein tyrosine kinases
- Epidermal growth factor receptor tyrosine kinase inhibitors: simple drugs with a complex mechanism of action?
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