Catalog Number | LT8803 |
Product Name | TNO211 MMP FRET Substrate, DABCYL-(gamma-Abu)-PQGL-E(EDANS)AK-NH2 |
Product Quantity | 1 mg |
Sequence | DABCYL-(γ-Abu)-PQGL-E(EDANS)AK-NH2 |
Purity | ≥95% |
Scientific Background | TNO211 is an internally quenched peptide substrate designed for matrix metalloproteinase assays. Its PQGL-containing protease-recognition region is flanked by a DABCYL quencher and an EDANS fluorophore. Cleavage disrupts intramolecular quenching and produces a fluorescence increase, allowing continuous measurement of MMP-dependent proteolysis. |
Research Applications | - MMP activity assays
- FRET protease measurements
- MMP inhibitor screening
- metalloproteinase assay development
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Experimental Notes | This is a reporter-modified substrate and should be treated as chemically distinct from unlabeled PQGL peptides. Protect from light and validate enzyme selectivity under the intended assay conditions. |
Selected References | - Fluorogenic substrates for matrix metalloproteinases and related protease assays
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