Tissue Kallikrein Fluorogenic Substrate, H-D-Val-Leu-Arg-AFC

H-D-Val-Leu-Arg-AFC is a fluorogenic peptide substrate used for tissue/glandular kallikrein activity measurements. Cleavage after the arginine residue releases AFC and generates a fluorescence signal. Published inhibitor-selectivity studies have used this exact substrate to quantify human tissue kallikrein activity and distinguish tissue kallikrein from plasma kallikrein under defined conditions.

• tissue kallikrein activity assays
• kallikrein inhibitor screening
• kinin-system protease studies
• fluorogenic serine-protease assays

H-D-Val-Leu-Arg-AFC can be hydrolyzed by related serine proteases, including plasma kallikrein-like activities under some conditions. Use purified enzymes or selective inhibitors when assigning specificity.

1. Involvement of tissue kallikrein but not plasma kallikrein in the development of symptoms mediated by endogenous kinins in acute pancreatitis in rats