SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein (LTP10051)

Product Name
SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein (LTP10051)
Catalog Number
LTP10051
Quantity
100ug
Price
$496 In stock
Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Description
Recombinant SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H).
Target
Spike S1 (Omicron BA.4/BA.5/BA.5.2)
Species
SARS-COV-2
Gene/Protein ID
QHD43416.1
Exact Sequence
Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H)
Molecular Weight
The protein has a predicted MW of 75.76 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) , His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 53.4ng/ml determined by ELISA. See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein (LTP10051)
  • 5 Units in Stock
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