SARS-COV-2 Spike S1 Protein (LTP10949)

Product Name
SARS-COV-2 Spike S1 Protein (LTP10949)
Catalog Number
LTP10949
Quantity
100ug
Price
$450 In stock
Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Description
Recombinant SARS-COV-2 Spike S1 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal. It contains Gln14-Arg683.
Target
Spike S1
Species
SARS-COV-2
Gene/Protein ID
QHD43416.1
Exact Sequence
Gln14-Arg683
Molecular Weight
The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on the Tris-Bis PAGE result.
Activity
The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
C-hFc-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
SARS-COV-2 Spike S1 Protein (LTP10949)
  • 5 Units in Stock
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