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| SARS-COV-2 Spike S1 (D614G) Protein (LTP10702) |
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| LTP10702 |
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| 100ug |
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$496 In stock |
| The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
| Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln14-Arg683(D614G). |
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| Spike S1 (D614G) |
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| SARS-COV-2 |
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| QHD43416.1 |
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| Gln14-Arg683(D614G) |
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| The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on the Tris-Bis PAGE result. |
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| Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 23.7ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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