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| SARS-Cov-2 Spike RBD (B.1.640.2/IHU) Protein (LTP10175) |
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| LTP10175 |
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| 100ug |
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$450 In stock |
| The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| Recombinant SARS-Cov-2 Spike RBD(B.1.640.2/IHU) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y). |
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| Spike RBD (B.1.640.2/IHU) |
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| SARS-COV-2 |
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| QHD43416.1 |
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| Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y) |
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| The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on the Tris-Bis PAGE result. |
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| Immobilized SARS-Cov-2 Spike RBD (B.1.640.2/IHU) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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