Catalog Number | LT8916 |
Product Name | S3 Fragment, ADF/Cofilin, LIMK1 Substrate |
Product Quantity | 1 mg |
Sequence | MASGVMVSDDVVKVFN |
Molecular Weight | 1698.1 |
Formula | C73H120N18O24S2 |
Purity | ≥95% |
Scientific Background | MASGVMVSDDVVKVFN contains the amino-terminal Ser3 phosphorylation region of Xenopus ADF/cofilin, a physiologically important target of LIM kinase. LIMK-mediated phosphorylation of cofilin at Ser3 suppresses its actin-depolymerizing activity and is a central regulatory step in actin-cytoskeleton remodeling. The S3 fragment has also been used as a competitive LIMK1 inhibitor in studies of growth-cone guidance and cytoskeletal signaling. |
Research Applications | - LIMK1 activity and substrate studies
- ADF/cofilin phosphorylation research
- actin-cytoskeleton signaling
- competitive LIMK1 inhibition studies
|
Experimental Notes | This peptide can function both as a sequence-defined LIMK phosphorylation-region reagent and as a competitive inhibitor depending on assay design. Biological conclusions should distinguish direct phosphorylation measurements from competition experiments. |
Selected References | - BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin
|
