Description:Recombinant SARS-CoV-2 Spike S1 Protein
Product: Recombinant protein of SARS-CoV-2 Spike S1 from Wuhan pneumonia virus (MN908947.3) , with a polyhistidine tag.
Cat #: LTP-V006
Host: Human cells
RefSeq: NC_045512.2/MN908947.3; YP_009724390.1/QHD43416.1; GeneID: 43740568;
Molecular Weight: Predicted MW of this product is ~78 kDa (16aa-685aa). However, it runs higher than 100 kDa on the reduce SDS-PAGE due to a post-translational modification when expressing in mammalian cells.
Applications: Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies; For research & development use only!
Background:The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV-2 spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV-2 (2019-nCoV) as in recent COVID-19 outbreak.
Purity: >90% by SDS-PAGE gel and Coomassie Blue staining
Formulation: Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives
Download Data File:
The S1 RBD binds to the ACE2 enzyme, using the Fc only as a negative control, which the RBD did not bind to. The assay was performed with an ELISA, detecting the RBD with streptavidin HRP.