Protein Kinase C Substrate

Protein Kinase C Substrate is a synthetic research peptide in the protein kinase C peptide substrate family. Protein kinase C isoforms recognize basic Ser/Thr-containing phosphorylation motifs. Synthetic peptide substrates derived from natural phosphorylation sites have been used to compare substrate specificity among PKC alpha, beta, and gamma isoforms and to quantify kinase activity under defined conditions.

• PKC activity assays
• Ser/Thr phosphorylation studies
• PKC isoform substrate-specificity studies
• kinase inhibitor screening

The exact sequence, reporter group, phosphorylation state, stereochemistry, and terminal modifications should be considered when comparing this reagent with the parent peptide or published assay substrates. Enzyme concentration, buffer composition, cofactors, pH, ionic strength, and substrate concentration can also affect measured activity.

1. Protein kinase C alpha, beta and gamma peptide substrate specificity