Catalog Number | LT8915 |
Product Name | pp60(v-SRC) Autophosphorylation Site, Phosphorylated |
Product Quantity | 1 mg |
Sequence | RRLIEDNE-pY-TARG |
Molecular Weight | 1672.8 |
Formula | C66H110N23O26P |
Purity | ≥95% |
Scientific Background | RRLIEDNE-pY-TARG is a phosphotyrosine-containing peptide corresponding to the autophosphorylation region of the Rous sarcoma virus transforming protein pp60(v-Src). Phosphorylation of the analogous activation-loop tyrosine is a hallmark of Src-family kinase activation. This defined phosphopeptide has been used in Src signaling and phosphatase studies and provides a useful reference for phosphotyrosine-recognition and dephosphorylation assays. |
Research Applications | - Src-family phosphotyrosine studies
- protein tyrosine phosphatase assays
- SH2/phosphotyrosine recognition research
- phosphopeptide analytical standards
|
Experimental Notes | The peptide is already phosphorylated and is therefore most appropriate as a phosphopeptide reference, binding ligand or phosphatase substrate. It should not be confused with the unphosphorylated RRLIEDNEYTARG Src kinase substrate. |
Selected References | - Epidermal growth factor stimulates the phosphorylation of synthetic tyrosine-containing peptides by A431 cell membranes
- Involvement of pp60c-src with two major signaling pathways in human breast cancer
- Combating trastuzumab resistance by targeting SRC, a common node downstream of multiple resistance pathways
|
