p70 S6 Kinase Selective Substrate, KKRNRTLTV

KKRNRTLTV is a synthetic peptide developed as a relatively selective substrate for p70 ribosomal S6 kinase. In the original specificity study, this sequence showed approximately 50-fold higher catalytic efficiency with p70 S6 kinase than with MAPKAP kinase-1, making it useful for comparative kinase assays and p70 S6 kinase activity measurements.

• p70 S6 kinase activity assays
• S6 kinase specificity studies
• kinase inhibitor screening
• phosphorylation kinetics

The reported selectivity is relative rather than absolute. Kinase identity should be supported by purified-enzyme experiments, selective inhibitors, or orthogonal pathway controls.

1. Comparison of the specificities of p70 S6 kinase and MAPKAP kinase-1 identifies a relatively specific substrate for p70 S6 kinase