Catalog Number | LT8897 |
Product Name | Neurogranin (28-43), Biotin-LC, PKC Substrate |
Product Quantity | 5 mg |
Sequence | Biotin-LC-AAKIQASFRGHMARKK |
Molecular Weight | 2139.7 |
Formula | C94H159N31O20S2 |
Purity | ≥95% |
Scientific Background | Biotin-LC-AAKIQASFRGHMARKK is a biotinylated derivative of the neurogranin 28-43 phosphorylation region, a well-characterized protein kinase C (PKC) substrate. The neurogranin sequence contains a central serine in a basic recognition context, while the long-chain biotin linker provides an affinity handle for streptavidin capture, immobilization and assay detection. This format is useful when PKC phosphorylation is coupled to solid-phase or affinity-based workflows. |
Research Applications | - PKC activity assays
- neurogranin phosphorylation studies
- streptavidin capture and immobilization
- biotin-based kinase assay development
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Experimental Notes | Biotin-LC adds a substantial affinity tag and spacer relative to native neurogranin 28-43. Kinetic values measured with the unconjugated peptide should not be assumed to be identical for the biotinylated form. |
Selected References | - Conformation of a Protein Kinase C Substrate, NG(28-43), and Its Analog in Aqueous and Sodium Dodecyl Sulfate Micell Solutions
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