Myristoylated PKC-zeta Pseudosubstrate Peptide (ZIP)

Myr-SIYRRGARRWRKL is a myristoylated pseudosubstrate-derived peptide widely known as zeta inhibitory peptide (ZIP). It has been used to inhibit PKMzeta/PKCzeta-related signaling in biochemical and neuronal studies. Later work showed that ZIP can interact with multiple PKC family isoforms and disrupt conventional PKC targeting, so it is best treated as a potent PKC-family perturbation reagent rather than an absolutely PKCzeta-specific inhibitor.

• PKC-family signaling studies
• pseudosubstrate inhibition experiments
• neuronal signaling research
• PKC isoform comparison studies

The N-terminal myristoyl group promotes membrane association and cellular uptake. Published evidence indicates broader PKC-family activity, so isozyme specificity should be established experimentally.

1. Protein kinase C zeta pseudosubstrate inhibitor peptide promiscuously binds PKC family isoforms and disrupts conventional PKC targeting and translocation
2. Matching biochemical and functional efficacies confirm ZIP as a potent competitive inhibitor of PKMzeta in neurons