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| Mouse FLT3 Ligand Protein (LTP10307) |
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| LTP10307 |
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| 100ug |
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$473 In stock |
| Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony-stimulating factors and interleukins. |
| Recombinant Mouse FLT3 Ligand Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Gly27-Gln189. |
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| FLT3 Ligand |
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| Mouse |
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| P49772-1 |
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| Gly27-Gln189 |
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| The protein has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on the Tris-Bis PAGE result. |
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| The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). See the testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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A. Active Recombinant Mouse Flt3 ligand/Flt3L Protein was determined by SOS-PAGE with Coomassie Blue, showing a band at 56 kDa;
B. Immobilized recombinant Human FLT3 at 0.5 ug/mL (700 uL/well) can bind FL T3L with a linear range of 0.05-1.3 ng/ml;
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