MMP-3 Selective FRET Substrate NFF-3

NFF-3 is an internally quenched fluorogenic substrate designed to discriminate stromelysin-1 (MMP-3) activity from several related MMPs. Published kinetic studies reported rapid hydrolysis by MMP-3, substantially slower cleavage by MMP-9, and little or no significant hydrolysis by MMP-1 or MMP-2 under the tested conditions. This makes NFF-3 especially useful for comparative MMP-3 activity measurements.

• MMP-3 activity assays
• MMP subtype discrimination
• MMP inhibitor screening
• cell- and conditioned-media protease studies

1. Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin 1
2. Comparison of metalloproteinase protein and activity profiling

This sequence-defined peptide is intended for research use. Solubility, adsorption, aggregation, stability and biological response can vary with concentration, pH, ionic strength, formulation, terminal chemistry and assay design. Validate reconstitution, storage and assay-specific controls experimentally.