MMP FRET Substrate 1, Mca-PLGL-Dap(Dnp)-AR-NH2

Mca-PLGL-Dap(Dnp)-AR-NH2 is an internally quenched fluorogenic substrate developed for continuous matrix metalloproteinase assays. The Mca fluorophore is quenched by the Dnp group before cleavage; proteolysis at the Gly-Leu region separates the reporter and quencher and produces a strong fluorescence increase. The substrate has been characterized with several human MMPs and is useful as a sensitive general MMP activity reagent.

• continuous MMP activity assays
• MMP-7/-8/-9/-13 comparative studies
• MMP inhibitor screening
• fluorogenic protease assay development

1. A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases

This sequence-defined peptide is intended for research use. Solubility, adsorption, aggregation, stability and biological response can vary with concentration, pH, ionic strength, formulation, terminal chemistry and assay design. Validate reconstitution, storage and assay-specific controls experimentally.