MAP Kinase Substrate; EGF-R (661-681)

MAP Kinase Substrate; EGF-R (661-681) is a synthetic research peptide in the synthetic protein-kinase peptide substrate family. Synthetic peptide substrates provide defined, reproducible reagents for measuring protein kinase activity and examining sequence requirements for phosphorylation. The exact kinase preference of a peptide depends on the phosphoacceptor residue and surrounding recognition motif; the stated source region and sequence should therefore guide assay selection.

• protein kinase activity assays
• phosphorylation kinetics
• substrate-specificity studies
• kinase inhibitor screening

The exact sequence, reporter group, phosphorylation state, stereochemistry, and terminal modifications should be considered when comparing this reagent with the parent peptide or published assay substrates. Enzyme concentration, buffer composition, cofactors, pH, ionic strength, and substrate concentration can also affect measured activity.

1. Aurora-A site specificity studied with synthetic peptide substrates