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| Human TNFR2/CD120b/TNFRSF1B Protein (LTP10953) |
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| LTP10953 |
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| 100ug |
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$360 In stock |
| Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. |
| Recombinant Human TNF R2/CD120b/TNFRSF1B Protein is expressed from Expi293 with mFc (IgG1) tag at the C-terminal. It contains Leu23-Asp257. |
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| TNFR2/CD120b/TNFRSF1B |
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| Human |
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| P20333 |
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| Leu23-Asp257 |
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| The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human TNFR2, mFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-mFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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