Human ROBO4 Protein (LTP10457)

Product Name
Human ROBO4 Protein (LTP10457)
Catalog Number
LTP10457
Quantity
100ug
Price
$406 In stock
Background
Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.
Description
Recombinant Human ROBO4 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Ala27-Glu469.
Target
ROBO4
Species
Human
Gene/Protein ID
Q8WZ75-1
Exact Sequence
Ala27-Glu469
Molecular Weight
The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human ROBO4 Protein (LTP10457)
  • 5 Units in Stock
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