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| Human ROBO4 Protein (LTP10457) |
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| LTP10457 |
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| 100ug |
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$406 In stock |
| Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis. |
| Recombinant Human ROBO4 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Ala27-Glu469. |
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| ROBO4 |
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| Human |
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| Q8WZ75-1 |
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| Ala27-Glu469 |
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| The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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