Human IL-7 Protein (LTP11015)

Product Name
Human IL-7 Protein (LTP11015)
Catalog Number
LTP11015
Quantity
100ug
Price
$316 In stock
Background
Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7R? or ? c chain, the two subunits that constitute the functional IL7 receptor.
Description
Recombinant Human IL-7 Protein is expressed from E.coli without tag. It contains Asp26-His177.
Target
IL-7
Species
Human
Gene/Protein ID
P13232
Exact Sequence
Asp26-His177
Molecular Weight
The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result.
Activity
Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml determined by ELISA. The affinity constant of 22.66 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
No Tag
Express System
E.coli
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8).

Image
Human IL-7 Protein (LTP11015)
  • 5 Units in Stock
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