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| Human IL-6 R alpha/CD126 Protein (LTP10837) |
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| LTP10837 |
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| 100ug |
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$473 In stock |
| The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed. |
| Recombinant Human IL-6 R alpha/CD126 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu20-Pro365. |
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| IL-6R alpha/CD126 |
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| Human |
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| P08887-1 |
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| Leu20-Pro365 |
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| The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human IL-6, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.25ug/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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