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| Human IL-1 Beta/IL-1F2 Protein (LTP10690) |
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| LTP10690 |
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| 100ug |
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$406 In stock |
| Interleukin-1 beta (IL-1?) is induced by inflammatory signals in a broad number of immune cell types. IL-1? (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
| Recombinant Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-terminal. It contains Ala117-Ser269. |
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| IL-1 Beta/IL-1F2 |
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| Human |
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| P01584 |
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| Ala117-Ser269 |
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| The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result. |
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| Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.44ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| E.coli |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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