| Human GPA33/A33 Protein (LTP10825) |
| LTP10825 |
| 100ug |
|
$339 In stock |
| Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling. |
| Recombinant Human GPA33/A33 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ile22-Val235. |
| GPA33/A33 |
| Human |
| Q99795-1 |
| Ile22-Val235 |
| The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on the Tris-Bis PAGE result. |
| Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. The affinity constant of 0.69 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| C-His-Avi |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| |