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| Human GDF15 Protein (LTP10238) |
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| LTP10238 |
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| 100ug |
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$473 In stock |
| Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15. |
| Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-terminal. It contains Ala197-Ile308. |
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| GDF15 |
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| Human |
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| Q99988-1 |
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| Ala197-Ile308 |
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| The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA. The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-His |
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| E.coli |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
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