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| Human DNAM-1/CD226 Protein (LTP10758) |
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| LTP10758 |
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| 100ug |
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$305 In stock |
| DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. |
| Recombinant Human DNAM-1/CD226 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu19-Asn247. |
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| DNAM-1/CD226 |
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| Human |
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| Q15762 |
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| Glu19-Asn247 |
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| The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human DNAM-1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 0.43ug/ml determined by ELISA (QC Test). The affinity constant of 0.22uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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