|
| Human CD40 Ligand/TNFSF5 Trimer Protein (LTP10763) |
|
| LTP10763 |
|
| 100ug |
|
|
$406 In stock |
| CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, ?5?1 integrin and ?IIb?3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. . |
| Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from Expi293 with monomeric hFc tag at the N-terminal. It contains Met113-Leu261. |
|
| CD40 Ligand/TNFSF5 Trimer |
|
| Human |
|
| P29965 |
|
| Met113-Leu261 |
|
| The protein has a predicted MW of 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Human CD40, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test). See testing image for detail. |
|
| N-monomeric hFc |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
