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| Human CD40 Ligand/TNFSF5 Trimer Protein (LTP10762) |
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| LTP10762 |
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| 100ug |
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$406 In stock |
| CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, ?5?1 integrin and ?IIb?3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. |
| Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal. It contains Met113-Leu261. |
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| CD40 Ligand/TNFSF5 Trimer |
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| Human |
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| P29965 |
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| Met113-Leu261 |
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| The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD40 Ligand Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 1.29ug/ml determined by ELISA. See testing image for detail. |
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| N-His-Flag |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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