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| Human CD300A Protein (LTP10484) |
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| LTP10484 |
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| 100ug |
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$450 In stock |
| CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation. |
| Recombinant Human CD300A Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Leu18-Pro180. |
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| CD300A |
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| Human |
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| Q9UGN4-1 |
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| Leu18-Pro180 |
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| The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD300A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD300A Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA. See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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