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| Human BDCA-2 Protein (LTP10341) |
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| LTP10341 |
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| 100ug |
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$473 In stock |
| BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling. |
| Recombinant Human BDCA-2 Protein is expressed from Expi293 with hFc tag at the N-terminal. It contains Phe46-Ile213. |
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| BDCA-2 |
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| Human |
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| Q8WTT0-1 |
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| Phe46-Ile213 |
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| The protein has a predicted MW of 47.1 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human BDCA-2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail. |
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| N-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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