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| Human B7-2/CD86 Protein (LTP11025) |
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| LTP11025 |
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| 100ug |
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$339 In stock |
| B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
| Recombinant Human B7-2/CD86 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu26-Pro247. |
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| B7-2/CD86 |
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| Human |
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| P42081-1 |
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| Leu26-Pro247 |
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| The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human B7-2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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