Catalog Number | LT8801 |
Product Name | HIV-1 Protease FRET Substrate I |
Product Quantity | 1 mg |
Sequence | DABCYL-GABA-SQNYPIVQ-EDANS |
CAS Number | 127134-13-8 |
Molecular Weight | 1532.8 |
Formula | C73H97N17O18S |
Purity | ≥95% |
Scientific Background | DABCYL-GABA-SQNYPIVQ-EDANS is an internally quenched fluorogenic substrate for HIV-1 protease. The peptide is derived from a natural retroviral processing-site sequence and is cleaved at the Tyr-Pro bond. Proteolysis separates DABCYL from EDANS, producing a fluorescence increase that enables continuous kinetic measurements of HIV-1 protease activity and inhibitor effects. |
Research Applications | - HIV-1 protease activity assays
- retroviral protease kinetics
- HIV protease inhibitor screening
- continuous FRET protease assays
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Experimental Notes | The substrate is commonly dissolved initially in concentrated DMSO because of limited aqueous solubility. Protect the fluorescent substrate from light and avoid repeated freeze-thaw cycles after reconstitution. |
Selected References | - Fluorescence-based continuous assay for the aspartyl protease of human immunodeficiency virus-1
- Sensitive, hydrosoluble, macromolecular fluorogenic substrates for human immunodeficiency virus 1 proteinase
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