Catalog Number | LT8955 |
Product Name | HIV-1 Protease FRET Substrate, HiLyte Fluor 488/QXL520 |
Product Quantity | 0.1 mg |
Sequence | QXL®520-GABA-SQNYPIVQ-K(HiLyte™ Fluor 488)-NH2 |
Molecular Weight | 2008.2 |
Purity | ≥95% |
Scientific Background | QXL520-GABA-SQNYPIVQ-K(HiLyte Fluor 488)-NH2 is a long-wavelength FRET substrate for HIV-1 protease. The SQNYPIVQ sequence derives from the p17/p24 cleavage junction of the HIV Gag precursor, an essential viral maturation site. The HiLyte Fluor 488/QXL520 pair shifts detection to approximately 490/520 nm, reducing short-wavelength autofluorescence and providing a sensitive continuous readout of HIV protease activity. |
Research Applications | - HIV-1 protease activity assays
- antiretroviral protease inhibitor screening
- viral polyprotein-processing research
- continuous FRET protease measurements
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Experimental Notes | This reporter construct is chemically distinct from EDANS/DABCYL HIV protease substrates even though the underlying cleavage motif is related. Kinetic and inhibitor data should be compared using matched substrate chemistry. |
Selected References | - Crystal structure of a complex of HIV-1 protease with a substrate-based inhibitor
- HIV protease as a target for the design of antiviral agents
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