Catalog Number | LT9028 |
| Product Name | Histone H3 (21-44) K27Ac-GK(Biotin)-NH2 |
| Product Quantity | 1 mg |
| Sequence | ATKAAR-K(Ac)-SAPSTGGVKKPHRYRPG-GK(Biotin)-NH2 |
| Molecular Weight | 2974.6 |
| Purity | ≥95% |
| Scientific Background | H3K27ac is a prominent active-chromatin mark associated with active promoters and enhancers and can oppose Polycomb-associated repression. This H3 tail peptide contains site-specific Lys27 acetylation together with a C-terminal GK(Biotin)-NH2 handle, providing an immobilizable reagent for acetyl-lysine reader, histone-binding and chromatin-regulation studies. |
| Research Applications | - H3K27ac reader-protein binding
- bromodomain studies
- streptavidin pull-down assays
- chromatin and epigenetics research
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| Experimental Notes | LifeTein identifies this reagent as residues 21-44, although its displayed sequence contains the H3 tail through RPG followed by GK(Biotin)-NH2. The exact vendor sequence, biotin handle and C-terminal amide are retained here. It is chemically distinct from free-acid H3K27ac constructs. |
| Selected References | - CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing
- Binding of the histone chaperone ASF1 to the CBP bromodomain promotes histone acetylation
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