| Product Name | Histone H1-Derived Peptide |
| Product Quantity | 1 mg |
| Sequence | GGGPATPKKAKKL |
| Molecular Weight | 1252.6 |
| Purity | ≥95% |
| Mechanism & Biological Significance | GGGPATPKKAKKL is a histone H1-derived proline-directed kinase substrate centered on a Thr-Pro motif. The sequence is useful for interrogating cyclin-dependent-kinase substrate recognition and for kinase-assay development in which phosphorylation of the central threonine is monitored. |
| Published Research Context | The exact 13-residue sequence was tested directly in a peer-reviewed substrate-finder study of the Leishmania CRK3:CYC6 cyclin-dependent kinase complex. It produced one of the strongest fluorescence-polarization responses among the screened serine/threonine kinase substrates, supporting direct use of this exact peptide as a CDK-family phosphorylation substrate. The sequence has also been incorporated as an exact histone-H1-derived substrate in engineered kinase studies. |
| Research Applications | - cyclin-dependent kinase substrate profiling
- kinase assay development
- phosphorylation and enzyme-kinetic studies
- comparative proline-directed kinase research
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| Experimental Considerations | Do not substitute the shorter H1P peptide PKTPKKAKKL or a FAM-labeled derivative when exact kinetic comparability is required. The 2010 Cdk5/p25 H1P paper used PKTPKKAKKL, not this 13-mer, so it is not used here as exact-construct evidence. |
| Selected Scientific References | - High Throughput Screens Yield Small Molecule Inhibitors of Leishmania CRK3:CYC6 Cyclin-Dependent Kinase
- An engineered lantipeptide synthetase serves as a general leader peptide-dependent kinase
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