Histone H1-Derived Peptide, 5-FAM-Labeled

5-FAM-GGGPATPKKAKKL is a fluorescent histone H1-derived kinase substrate. The H1-derived motif can be phosphorylated by CDK2, CDK5 and PKA, while the 5-FAM reporter enables direct fluorescence detection around 494/521 nm in separation-based or other fluorescence-compatible assay formats.

• fluorescent CDK assays
• CDK5 kinase research
• PKA substrate assays
• kinase inhibitor screening

Protect the FAM-labeled peptide from light. The fluorophore is part of the exact chemical identity and can change chromatography and apparent enzyme kinetics relative to the unlabeled substrate.

1. Kinetic Mechanistic Studies of Cdk5/p25-Catalyzed H1P Phosphorylation: Metal Effect and Solvent Kinetic Isotope Effect
2. Studies on the mechanism of phosphorylation of synthetic polypeptides by a calf thymus cyclic AMP-dependent protein kinase