Catalog Number | LT8951 |
Product Name | Enterokinase Substrate, GDDDDK-βNA |
Product Quantity | 1 mg |
Sequence | GDDDDK-ßNA |
CAS Number | 70023-02-8 |
Molecular Weight | 788.8 |
Formula | C34H44N8O14 |
Purity | ≥95% |
Scientific Background | GDDDDK-βNA is a chromogenic/fluorogenic peptide substrate derived from the canonical enteropeptidase recognition motif Asp-Asp-Asp-Asp-Lys. Enteropeptidase, historically called enterokinase, initiates digestive zymogen activation by cleaving trypsinogen after this acidic Lys-containing sequence. The β-naphthylamide reporter provides a convenient analytical handle for measuring enteropeptidase activity and for studies of proenteropeptidase activation. |
Research Applications | - enteropeptidase/enterokinase activity assays
- digestive zymogen activation research
- serine-protease specificity studies
- enzyme inhibitor and assay-development studies
|
Experimental Notes | The substrate models the minimal acidic recognition motif rather than full-length trypsinogen. Cleavage rates depend on enzyme preparation, ionic conditions and substrate concentration, so use matched assay conditions for quantitative comparisons. |
Selected References | - Bovine Proenteropeptidase Is Activated by Trypsin, and the Specificity of Enteropeptidase Depends on the Heavy Chain
- Purification and Further Characterization of Enteropeptidase from Porcine Duodenum
|
