EGFR Tyr992 Phosphopeptide, 5-FAM Labeled

5-FAM-DADE-pY-LIPQQG is a fluorescent phosphotyrosine peptide derived from the Tyr992 autophosphorylation region of the epidermal growth factor receptor (EGFR). Because Tyr is already phosphorylated, this reagent is primarily a protein-tyrosine-phosphatase substrate rather than a kinase phosphate-acceptor peptide. LifeTein reports efficient dephosphorylation by mammalian PTP1B and Yersinia protein tyrosine phosphatase. The 5-FAM label provides a fluorescent handle for analytical and phosphatase-assay workflows.

• PTP1B activity assays
• protein tyrosine phosphatase studies
• EGFR Tyr992 phosphopeptide research
• fluorescence-based phosphopeptide assays

This peptide already contains phosphotyrosine. Do not market it as an unphosphorylated EGFR kinase substrate. Protect the FAM-labeled reagent from light and establish optical background controls for the intended assay.

1. A Continuous Spectrophotometric and Fluorometric Assay for Protein Tyrosine Phosphatase Using Phosphotyrosine-Containing Peptides
2. Molecular Basis for Substrate Specificity of Protein-tyrosine Phosphatase 1B