DNA-PK Substrate Peptide, EPPLSQEAFADLWKK

EPPLSQEAFADLWKK is a p53-derived peptide substrate used for DNA-dependent protein kinase (DNA-PK) activity measurements. The construct corresponds to the p53 11-24 region with substitutions that remove competing phosphoacceptor sites, providing a defined peptide reagent for studying DNA-PK-catalyzed phosphorylation. DNA-PK is a central kinase in DNA double-strand-break repair by non-homologous end joining.

• DNA-PK kinase activity assays
• DNA double-strand-break signaling research
• kinase inhibitor screening
• phosphorylation method development

The N-terminal glutamate can be susceptible to pyroglutamate formation under some conditions; storage and sample preparation should therefore be standardized for quantitative work.

1. Selective inhibition of the DNA-dependent protein kinase (DNA-PK) by the radiosensitizing agent caffeine