Bovine Beta-Casein Monophosphopeptide

FQ-pS-EEQQQTEDELQDK is a monophosphorylated peptide derived from bovine beta-casein and contains a defined phosphoserine residue. This phosphopeptide is widely used as an analytical reference in phosphopeptide enrichment and mass-spectrometry workflows. Published LC-MS/MS studies identify FQpSEEQQQTEDELQDK as a characteristic beta-casein phosphopeptide and have used it to evaluate phosphopeptide enrichment, recovery and phosphate-retention methods.

• phosphopeptide LC-MS/MS method development
• phosphopeptide enrichment evaluation
• phosphoserine analytical standards
• phosphoproteomics workflow controls

The phosphate group is an integral part of the analyte. Sample preparation and MS/MS conditions can influence phosphate retention and fragmentation, so comparisons should use matched analytical conditions.

1. An Initial Characterization of the Serum Phosphoproteome
2. Effect of Metals in Biomimetic Dimetal Complexes on Affinity and Gas-Phase Protection of Phosphate Esters