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| Biotinylated Human VEGF165 Protein (LTP10880) |
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| LTP10880 |
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| 100ug |
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$789 In stock |
| Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
| Recombinant Biotinylated Human VEGF165 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala27-Arg191. |
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| VEGF165 |
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| Human |
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| P15692-4 |
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| Ala27-Arg191 |
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| The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on the Tris-Bis PAGE result. |
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| Immobilized Anti-VEGF165 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 32.8ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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