biotin-Ahx-PPPPRGDRGDRGD-NH2

This peptide contains multiple repeats of the RGD (Arg-Gly-Asp) motif, which is recognized by integrins, a family of cell surface receptors involved in cell adhesion and signaling. The RGD sequence is commonly found in extracellular matrix (ECM) proteins such as fibronectin, vitronectin, and fibrinogen, and plays a crucial role in mediating cell attachment to these matrices.

The PPPPRGDRGDRGD peptide is utilized in various biomedical and research applications:

• Cell Adhesion Studies: Investigating how cells interact with ECM proteins and the role of integrins in these processes.
• Tissue Engineering: Incorporating into biomaterials to enhance cell attachment and proliferation, which is crucial for tissue engineering and regenerative medicine.
• Drug Delivery: Targeting drugs to specific cells, particularly in cancer therapy, by exploiting the overexpression of certain integrins on tumor cells.
• Diagnostic Imaging: Conjugating with imaging agents to visualize tumors and other pathological conditions where integrins are overexpressed.
• Wound Healing: Promoting cell migration and tissue repair by enhancing cell adhesion at wound sites.

biotin-Ahx-PPPPRGDRGDRGD-NH2 is a 15-residue synthetic peptide with the sequence Pro-Pro-Pro-Pro-Arg-Gly-Asp-Arg-Gly-Asp-Arg-Gly-Asp-Asn-His. Biotin provides an affinity handle for streptavidin-based capture or detection; the Ahx spacer separates the peptide from an attached label or affinity handle; C-terminal amidation removes the terminal carboxylate charge. The sequence is enriched in basic residues (0 Lys and 3 Arg), giving the sequence a cationic character under many aqueous conditions; is rich in Gly/Pro residues, a composition that can influence local backbone flexibility and conformational preferences. No specific receptor, enzyme, pathway, disease association, or biological activity is assigned to this peptide without product-specific experimental evidence.

• streptavidin-based capture or detection
• binding and pull-down assay development
• LC-MS/HPLC analytical method development
• sequence-specific assay controls

Sequence-derived chemical properties are provided to support reagent selection and experimental planning. They do not establish a biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions.