5-FAM-PMDM6 Fluorescent MDM2-Binding Probe

5-FAM-PMDM6 is a fluorescent p53-mimetic probe used to measure binding at the MDM2 p53-recognition pocket. Its constrained noncanonical residues and phosphotyrosine-containing design support sensitive direct and competitive fluorescence-polarization assays.

• MDM2 binding and competition assays
• Fluorescence-polarization inhibitor screening
• p53-MDM2 interaction research
• Assay optimization and probe validation

Fluorescence measurements are sensitive to probe concentration, polarization settings, plate material, buffer composition and nonspecific adsorption. Protect the labeled peptide from light and validate assay-specific controls.

1. Structure-based design of MDM2-p53 inhibitors
2. Fluorescence-polarization MDM2 binding assays using PMDM6-F