520 MMP FRET Substrate 14, FAM/QXL520

520 MMP FRET Substrate 14, FAM/QXL520 is an internally quenched peptide designed for fluorescence-resonance-energy-transfer measurements of matrix metalloproteinase activity. The peptide combines an MMP-sensitive recognition sequence with the 5-FAM/QXL 520 reporter pair. In the intact molecule, QXL 520 suppresses 5-FAM fluorescence; proteolytic cleavage separates the reporter pair and restores green fluorescence near 494/521 nm. The verified LifeTein activity profile includes MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-12 and MMP-13. MMPs participate in extracellular-matrix turnover and also process signaling proteins, receptors, cytokines and other bioactive molecules.

• matrix metalloproteinase activity assays
• extracellular-matrix remodeling research
• MMP inhibitor screening
• fluorescent protease assay development

This engineered substrate contains γ-aminobutyric acid, cyclohexylalanine, S-methyl-L-cysteine and diaminobutyric acid; these noncanonical residues are integral to the assay substrate. MMP peptide substrates generally show overlapping enzyme specificity, so activity in complex samples should be assigned with purified enzymes, selective inhibitors, or orthogonal controls. Protect the FAM/QXL 520 reagent from light.

1. Matrix Metalloproteinases: Structure, Function, and Their Exploitation as Therapeutic Targets in Cancer
2. Matrix Metalloproteinases in Health and Disease