Catalog Number | LT8961 |
Product Name | 520 MMP FRET Substrate 13, FAM/QXL520 |
Product Quantity | 0.1 mg |
Sequence | 5-FAM-RPKPVE-Nva-WRK(QXL® 520)-NH2 |
Molecular Weight | 2144.4 |
Purity | ≥95% |
Scientific Background | 520 MMP FRET Substrate 13, FAM/QXL520 is an internally quenched peptide designed for fluorescence-resonance-energy-transfer measurements of matrix metalloproteinase activity. The peptide combines an MMP-sensitive recognition sequence with the 5-FAM/QXL 520 reporter pair. In the intact molecule, QXL 520 suppresses 5-FAM fluorescence; proteolytic cleavage separates the reporter pair and restores green fluorescence near 494/521 nm. The verified LifeTein activity profile includes MMP-3 and MMP-12. MMPs participate in extracellular-matrix turnover and also process signaling proteins, receptors, cytokines and other bioactive molecules. |
Research Applications | - matrix metalloproteinase activity assays
- extracellular-matrix remodeling research
- MMP inhibitor screening
- fluorescent protease assay development
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Experimental Notes | This substrate incorporates norvaline and has a verified activity profile focused on MMP-3 and MMP-12. MMP peptide substrates generally show overlapping enzyme specificity, so activity in complex samples should be assigned with purified enzymes, selective inhibitors, or orthogonal controls. Protect the FAM/QXL 520 reagent from light. |
Selected References | - Matrix Metalloproteinases: Structure, Function, and Their Exploitation as Therapeutic Targets in Cancer
- Matrix Metalloproteinases in Health and Disease
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