Catalog Number | LT8802 |
Product Name | 490 MMP FRET Substrate X, DABCYL-RPLALWRS-EDANS |
Product Quantity | 1 mg |
Sequence | DABCYL-RPLALWRS-EDANS |
Molecular Weight | 1497.8 |
Formula | C73H100N20O13S |
Purity | ≥95% |
Scientific Background | DABCYL-RPLALWRS-EDANS is an internally quenched fluorogenic peptide substrate for matrix metalloproteinase activity. The intact construct places the DABCYL quencher near the EDANS fluorophore; proteolytic cleavage separates the pair and increases fluorescence. The RPLALWRS sequence has been used as a broad MMP-responsive substrate, making this reagent useful for comparative metalloproteinase activity measurements rather than as an absolutely isoform-specific substrate. |
Research Applications | - matrix metalloproteinase activity assays
- continuous FRET protease assays
- MMP inhibitor screening
- comparative MMP specificity studies
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Experimental Notes | Protect the peptide from light. Reported optical properties for this DABCYL/EDANS construct are approximately 340 nm excitation/490 nm emission. Enzyme identity should be established with purified enzymes, selective inhibitors or orthogonal controls. |
Selected References | - Kinetic Analysis of Matrix Metalloproteinase Activity Using Fluorogenic Triple-Helical Substrates
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