Cy5 Labeled Peptides

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Cy5 Peptide Labeling

Cy5 is a widely used far-red cyanine dye for peptide labeling, microscopy, flow cytometry, and multicolor detection. It is a common choice for low-background far-red fluorescence applications.

Key Properties

Excitation / Emission646 / 662 nm
ColorFar-red
Laser Compatibility633 nm / 640 nm
Typical UseMicroscopy, peptide tracking, flow cytometry
NotesPopular far-red dye for low-background imaging
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Cy5 Spectrum

Cy5 excitation emission spectrum

Why Use Cy5?

  • Useful far-red signal for low-background detection
  • Commonly paired with Cy3 in multicolor studies
  • Compatible with 633 nm and 640 nm laser systems
  • Widely used for peptide imaging and tracking
Tip: Cy5 and Alexa Fluor 647 are often considered spectrally related far-red options for peptide conjugation projects.

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