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LifeTein provides custom polyclonal antibody services for peptide antigens, phosphopeptides, recombinant proteins, and related immunogens. The most popular format is our peptide-to-polyclonal antibody workflow, which combines peptide antigen design, peptide synthesis, carrier conjugation, immunization, and peptide affinity purification into one service path.
Our strongest advantage in this area is the close connection between peptide antigen design, peptide synthesis, and downstream antibody production. For many research projects, this allows a more coherent workflow than ordering peptide and antibody services separately. The final affinity purification step is especially important for peptide antibody projects because it helps enrich antibodies that specifically recognize the designed peptide antigen.
| Main service | Peptide antigen design → peptide synthesis → KLH conjugation → immunization → peptide affinity purification |
| Main advantage | Affinity-purified antibodies enriched against the same peptide sequence used for antigen design |
| Common use | Western blot, ELISA, IP, IF, IHC, phospho-site studies, and custom target validation |
| Typical species | Rabbit is the most commonly requested species; other species may also be available depending on project needs |
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Most important differentiator
For many customers, the key value is not just getting a polyclonal antibody—it is getting a peptide-driven antibody workflow in which the antigen design, peptide synthesis, and peptide affinity purification are all aligned.
References using synthetic peptides and antibodies from LifeTein. See search results page from the Google Scholar.
The polyclonal antibodies recognizing CENH3 protein and kinetochore protein KNL1 were generated by LifeTein.
The polyclonal antibody recognizing human phosphorylated Ser-661(mouse Ser-656) in UHRF1 was generated by LifeTein, LLC.
Polyclonal rabbit antibodies to Ll-Bhp-1 were commercially produced using recombinant Ll-Bhp-1 and purified by protein A affinity chromatography (LifeTein, Somerset, NJ, USA).
LifeTein produced a series of snake venom antibodies. Venom species used for rabbit IgG production: Tiger, Brown, Black, Death adder, and Taipan. The snake venoms were used for immunization of rabbits to generate polyclonal antibodies.
Protein A purified rabbit IgG specific for each venom species (Table 1) were purchased from a commercial supplier (Lifetein, Somerset, NJ, USA).
LifeTein designed and synthesized a series of peptides: CENH3, MIS12 and NDC80. The peptides were used for immunization of rabbits to generate polyclonal antibodies.
... The peptide synthesis, immunization, and antibody purification were performed by LifeTein...
LifeTein generated Anti-gM and anti-gN antibodies for Vaccine Analytical Research Development and Vaccine Process Development Merck & Co., Inc., Kenilworth, NJ, USA. The rabbit polyclonal anti-gM and anti-gN antibodies were elicited by vaccinating animals with synthesized peptide sequences of gM (1-13aa and 345-372aa) and gN (61-101aa). Animal immunization and sera collection were performed at Lifetein, LLC (Hillsborough, NJ, USA). The sera were affinity-purified by its corresponding peptides.
LifeTein helped designed and synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific antibody productions. The phospo-specific antibodies by LifeTein were confirmed to react with the epidermal growth factor receptor (EGFR). The Hung's lab showed that AGO2-Y393 phosphorylation mediates EGFR-enhanced cell survival and invasiveness under hypoxia. These findings suggest that modulation of miRNA biogenesis is important for stress response in tumour cells.
... The following peptides were chemically synthesized for antibody production in mice (Lifetein), Elisa verification (LifeteinConc.) and peptide competition assay in immunohistochemistry (IHC)... Supplementary information
George R. Tiller, Potential Roles of Peroxidases in Caenorhabditis Elegans Innate Immunity. 2014
Custom peptide antibodies were provided by LifeTein to study SKPO-1. The SKPO-1 peroxidase domain peptide was synthesized by LifeTein. The polyclonal antibodies were raised in rabbits and then affinity purified to accomplish high specifications and concentrations. The antibodies were successfully used to localize the SKPO-1 protein in hypodermis. It was found that the peroxidase SKPO-1 contributes to the host immune response during infection.
... With respect to having a pAb raised for IHC, I designed a synthetic SKPO-1 peroxidase domain peptide to be chemically synthesized and have a pAb raised in rabbits against said peptide by Lifetein®. This endeavor was successful and is covered in Chapter 3...
LifeTein helped synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific p-METTL3 (S43) antibody productions. Sun et al. demonstrate that activation of the ERK pathway promotes m6A methylation by phosphorylation of METTL3 and WTAP.
LifeTein made the affinity-purified rabbit polyclonal anti-SclA antibody. The purified streptococcal collagen-like protein A (SclA) antibodies demonstrated that a mutation in Group A Streptococcus carrier strains increase adherence and decrease virulence.
LifeTein designed and synthesized a series of phosphorylated peptides. ELISA and dot blot analysis were performed for the verification of antibody specificity.
... Antibodies to the Thr345, Ser416, and Ser497 phosphorylation sites of XPO5 were generated in collaboration with Lifetein LLC... Supplementary information
