The SrtA substrates Biotin–aminohexanoic acid–LPETGS and SELPETGG were used to interact with immune cells ‘Labelling Immune Partnerships by SorTagging Intercellular Contacts’ (LIPSTIC). The peptide-receptor interactions enable the direct measurement of dynamic cell-cell interactions. The peptides are flexible tools with different receptor-ligand pairs and a range of detectable labels.

Schematic representation of the LIPSTIC approach

Nature, Monitoring T cell–dendritic cell interactions in vivo by intercellular enzymatic labeling

Peptide Biotin–aminohexanoic acid–LPETGS, SELPETGG was purchased from LifeTein.