The SrtA substrates Biotin–aminohexanoic acid–LPETGS and SELPETGG were used for the interactions between immune cells ‘Labelling Immune Partnerships by SorTagging Intercellular Contacts’ (LIPSTIC). The peptide-receptor interactions enable the direct measurement of dynamic cell–cell interactions. The peptides are flexible tools for use with different receptor–ligand pairs and a range of detectable labels.
Schematic representation of the LIPSTIC approach
Nature, Monitoring T cell–dendritic cell interactions in vivo by intercellular enzymatic labelling
Peptide Biotin–aminohexanoic acid–LPETGS, SELPETGG was purchased from LifeTein.
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