Monitoring T cell–dendritic cell interactions in vivo using labeled peptides

The SrtA substrates Biotin–aminohexanoic acid–LPETGS and SELPETGG were used for the interactions between immune cells ‘Labelling Immune Partnerships by SorTagging Intercellular Contacts’ (LIPSTIC). The peptide-receptor interactions enable the direct measurement of dynamic cell–cell interactions. The peptides are flexible tools for use with different receptor–ligand pairs and a range of detectable labels.

peptide-receptor interaction

Schematic representation of the LIPSTIC approach


Nature, Monitoring T cell–dendritic cell interactions in vivo by intercellular enzymatic labelling

Peptide Biotin–aminohexanoic acid–LPETGS, SELPETGG was purchased from LifeTein.

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